Cytochrome C抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質(zhì)保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實驗結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質(zhì)控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0013R
英文名稱Cytochrome C
中文名稱細胞色素C抗體
別 名CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4.
JournalPMIDIFApplicationFree Radical Biology and Medicine (2016)268787735.7360WBPLoS One (2014)255069323.5340WBEnvironmental Toxicology (2015)263067063.1970WBNeuroscience (2014)246992243.1220WBBrain research bulletin (2008)183956162.9350WBNeurochemical Research (2015)261234822.5930WBChemico-Biological Interactions (2016)267721552.5770WBEnvironmental and Molecular Mutagenesis (2014)253391312.5530WBToxicology and industrial health (2014)252272251.7100WBBiological Trace Element Research (2014)254312991.6080WBMolecular Medicine Reports (2015)256340591.4840WB
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領(lǐng)域腫瘤 心血管 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 脂蛋白 線粒體
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig,
Cytochrome C抗體產(chǎn)品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Cytochrome C抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Cytochrome C抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlCytochrome C抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
Cytochrome C抗體關(guān)聯(lián)產(chǎn)品列表：
bs-0033R YES 合格 P53 protein(wt-p53) 腫瘤抑制基因P53蛋白/野生型P53抗體
bs-0035R No 合格 CREB-1 環(huán)腺苷酸應答元件結(jié)合蛋白抗體
bs-0036R No 合格 phospho-CREB-1(Ser133) 磷酸化CREB-1抗體
bs-0037R YES 合格 合格 AIF 調(diào)亡誘導因子抗體
bs-0038R No 合格 GLP-1 (7-36) 胰高血糖素樣肽-1抗體
bs-0043R No 合格 合格 DFFB Caspase 激活的脫氧核糖核酸酶抗體
bs-0044R No 合格 BNP 腦鈉素/利鈉肽抗體
bs-0047R No 合格 合格 Insulin Receptor alpha 胰島素受體α抗體
bs-0049R YES 合格 合格 Caspase-9 活化半胱胺酸蛋白酶蛋白-9抗體
bs-0050R YES 合格 合格 合格 Caspase-9 活化半胱胺酸蛋白酶蛋白-9抗體
bs-0052R YES 合格 Caspase 8 半胱氨酸蛋白酶8抗體
bs-0057R No 合格 Amylin 糖尿病相關(guān)肽/胰島淀粉樣肽抗體
bs-0060R No 合格 合格 CEA 癌胚抗原抗體
bs-0061R YES 合格 合格 beta-Actin (Loading Control) β-肌動蛋白/β-Actin 抗體（內(nèi)參抗體）
bs-0064R YES 合格 Neurokinin 1 Receptor P物質(zhì)受體抗體