Nestin抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬(wàn)種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國(guó)75%以上現(xiàn)貨。
產(chǎn)品編號(hào)bs-8203R
英文名稱Nestin
中文名稱巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體
別 名ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
規(guī)格100ul 200ul
研究領(lǐng)域細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 細(xì)胞類型標(biāo)志物
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Cow,
Nestin抗體產(chǎn)品應(yīng)用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Nestin抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Nestin抗體溶解方法：
1、在我們公司購(gòu)買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無(wú)需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlNestin抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。
Nestin抗體關(guān)聯(lián)產(chǎn)品列表：
bs-0006R YES 合格 Nestin 巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體
bs-0008R YES 合格 合格 Nestin 巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體
bs-0009R No 合格 alpha-Synuclein 核突觸蛋白-α抗體（N端）
bs-0013R YES 合格 合格 Cytochrome C 細(xì)胞色素C抗體
bs-0014R YES 合格 IGF 1 胰島素樣生長(zhǎng)因子1抗體
bs-0016R YES 合格 合格 Tyrosine Hydroxylase 酪氨酸羥化酶抗體
bs-0017R No 合格 Galanin 甘丙肽/神經(jīng)節(jié)肽抗體
bs-0018R No 合格 IDE 胰島素降解酶抗體
bs-0019R YES 合格 合格 osteopontin 骨橋蛋白/分泌型磷蛋白1抗體
bs-0022R YES 合格 合格 ERK1 + ERK2 絲裂原活化蛋白激酶1/ERK 1/2抗體
bs-0023M No 合格 GSK-3 beta 糖原合酶激酶-3β抗體
bs-0024R No 合格 合格 presenilin 1 早老素蛋白-1抗體
bs-0026R YES 合格 osteopontin 骨橋蛋白/分泌型磷蛋白1抗體
bs-0027R YES 合格 Melatonin Receptor 1A 褪黑素受體1A/松果體素受體1A抗體
bs-0028R YES 合格 GSK-3 Beta 糖原合酶激酶-3β抗體
bs-0029R YES 合格 PP2A alpha + beta 蛋白質(zhì)磷酸酶-2A抗體
bs-0031R No 合格 合格 BTG2 B細(xì)胞遷移基因2抗體
bs-0032R YES 合格 合格 合格 Bcl-2 Bcl-2抗體
bs-0033R YES 合格 P53 protein(wt-p53) 腫瘤抑制基因P53蛋白/野生型P53抗體
bs-0035R No 合格 CREB-1 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體