如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: BL21 Star(DE3)大腸桿菌表達菌株
產(chǎn)品型號:
產(chǎn)品展商: XYbscience
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
BL21 Star(DE3)大腸桿菌表達菌株發(fā)貨前均經(jīng)過嚴格的多重驗證，如存在質(zhì)量問題，請在收到產(chǎn)品的三個月內(nèi)通知我司。BL21 Star(DE3)大腸桿菌表達菌株收到質(zhì)粒后請短暫離心，加入20μl ddH2O溶解質(zhì)粒，取2μl轉(zhuǎn)化至對應感受態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。
BL21 Star(DE3)大腸桿菌表達菌株
的詳細介紹
BL21 Star(DE3)大腸桿菌表達菌株
產(chǎn)品信息：
|
貨號
|
名稱
|
產(chǎn)品形式
|
規(guī)格
|
儲存
|
|
XY1488
|
BL21 Star(DE3) Strain
|
|
100μl
|
-20℃
|
使用說明：
信裕質(zhì)粒平臺的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴格的多重驗證，如存在質(zhì)量問題，請在收到產(chǎn)品三個月內(nèi)通知我司。收到甘油菌后，請及時在固體平板上劃線培養(yǎng)，挑取單菌落接種到液體培養(yǎng)基中，震蕩培養(yǎng)后使用并保存甘油菌。如需獲取其他詳細信息請登錄我司官網(wǎng)聯(lián)系QQ客服查詢。
基因型：
F- ompT hsdSB (rB-, mB-) gal dcm rne131 (DE3)
基本信息：
抗性：無
培養(yǎng)基：LB
菌株類別：大腸桿菌
培養(yǎng)條件：37℃，有氧，LB
質(zhì)粒轉(zhuǎn)化：42℃熱激
保存方式：20%甘油，-20℃
基本應用：用于蛋白表達
備注：
菌株簡介：
BL21 Star (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion? pET vectors). BL21 Star (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 108 cfu/μg plasmid DNA.
? A genotype that promotes high mRNA stability and protein yield
? Ideal for use with low copy number, T7 promoter-based plasmids
? The single tube, single use format allows all transformation steps to take Enhanced Expression of Non-Toxic Recombinant Proteins
BL21 Star (DE3) cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promotor, and IPTG is required to induce expression. This strain also offers enhanced mRNA stability due to a mutation in the RNaseE gene (rne131), which reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. Protein expression is further enhanced by the absence of the lon and outer membrane (OmpT) proteases, which reduces degradation of heterologous proteins.
The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes.
BL21 Star(DE3)來源于BL21(DE3)菌株, 含有rne131基因突變體，rne131突變基因能夠增強菌株細胞內(nèi)mRNA的穩(wěn)定性，從而有效提高蛋白表達能力。
主要適用于T7啟動子載體的高水平蛋白表達。
注意事項：
1、為了您的**和健康，請穿實驗服并戴一次性手套操作。本產(chǎn)品僅可用于實驗室研究，不能用于動物，人體以及作為食品添加劑等用途。
2、使用甘油菌時可不完全融解，在甘油菌表面蘸取少量涂布固體瓊脂平板即可，也可完全融解后使用，但隨著凍融次數(shù)的增加，菌株的活力會逐漸下降。