Cyclin B1 Rabbit Monoclonal Antibody
|
產品編號
|
產品名稱
|
產品包裝
|
產品價格
|
|
XY1606
|
Cyclin B1
Rabbit Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應性
|
分子量
|
|
Rabbit
|
WB, IP, IF,
IHC, ICC
|
H
|
55.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
1:10-1:50
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
-
|
-
|
-
|
抗體詳細信息如下:
|
About this
Antibody
|
|
Name
|
Cyclin B1
Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
891(Human)
|
|
SwissProt
|
P14635(Human)
|
|
Synonyms
|
CCNB
|
|
Category
|
Cell Cycle
|
|
Background
|
In eukaryotic
cells, mitosis is initiated following the activation of a protein kinase
known variously as maturation-promoting factor, M-phase specific histone
kinase or M-phase kinase. This protein kinase is composed of a catalytic
subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight
subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of
control, of which the regulation of the catalytic subunit by tyrosine
phosphorylation is the best understood. Tyrosine phosphorylation inhibits the
Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of
mitosis, directly activates the pre-MPF complex. Evidence has established
that B type cyclins not only act on M-phase regulatory subunits of the Cdc2
protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine
phosphatase, of which Cdc2 is the physiological substrate. The specificity of
this effect is shown by the inability of either cyclin A or cyclin D1 to
display any such stimulation of Cdc25A or Cdc25B.
|
包裝清單:
|
產品編號
|
產品名稱
|
包裝
|
|
XY1606
|
Cyclin B1
Rabbit Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
Cyclin B1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。