MKLP1 Rabbit Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價格
|
|
XY1537
|
MKLP1 Rabbit
Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IF, ICC
|
H, M, R
|
98.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
-
|
1:50-1:200
|
-
|
1:50-1:200
|
-
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this
Antibody
|
|
Name
|
MKLP1 Rabbit
Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
9493(Human);
71819(Mouse)
|
|
SwissProt
|
Q02241(Human);
E9Q5G3(Mouse); D4AAG4(Rat)
|
|
Synonyms
|
Kinesin-like
protein KIF23; Kinesin-like protein 5; Mitotic kinesin-like protein 1; KIF23;
KNSL5
|
|
Category
|
Cell Cycle
|
|
Background
|
The monoclonal
antibody CHO1 detects a spindle antigen required for mitotic progression.
Screening a HeLa cell cDNA expression library with this antibody has been
shown to yield a cDNA predicted to encode a protein significantly related
within its amino terminal half to the motor ends of members of the kinesin
superfamily. Since this similarity does not extend further, it has been
suggested that the CHO1 antigen, now designated MKLP-1 (mitotic kinesin-like
protein-1), represents a novel kinesin. Sequence analysis has also been shown
to predict that MKLP-1 possesses features typical of nuclear proteins.
Immunocytological studies have demonstrated that MKLP-1 moves from the
nucleus early in mitosis and then to the midbody XYter cytokinesis. MKLP-1
has been shown to bundle antiparallel microtubules in vitro and to move them
at rates comparable to spindle elongation in vivo. A hamster homolog of
MKLP-1, designated CHO1 antigen, has also been isolated. Although apparently
functionally equivalent with respect to microtubule bundling activity, there
are significant differences between the human and hamster proteins at their
C-termini, possibly due to alternative splicing or the presence of more than
one MKLP-1 locus.
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY1537
|
MKLP1 Rabbit
Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
MKLP1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。