Phospho-GSK-3α/β (Tyr216/279) Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1522
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Phospho-GSK-3α/β (Tyr216/279) Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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51.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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1:50-1:500
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1:50-1:200
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1:50-1:500
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Phospho-GSK-3α/β (Tyr216/279) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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2931/2932(Human);
606496/56637(Mouse); 50686/84027(Rat)
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SwissProt
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P49840/P49841(Human);
Q2NL51/Q9WV60(Mouse); P18265/P18266 (Rat)
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Synonyms
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Glycogen
synthase kinase 3 alpha
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Category
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PI3K/Akt/mTOR
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Background
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Glycogen
synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that
regulate metabolic enzymes and transcription factors, which are responsible
for coordinating processes such as glycogen synthesis and cell adhesion.
GSK-3β activity is also required for nuclear activity
of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through
differential phosphorylation of serine/threonine residues, which have an
inhibitory effect, and tyrosine residues, which have an activating effect.
Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser
21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein
kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals.
Additionally, GSK-3 physically associates with cAMP-dependent protein kinase
A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser
9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by
phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism,
neuronal cell development, and body pattern formation. Tyrosine
dephosphorylation of GSK-3 is involved in its extracellular signal-dependent
inactivation.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1522
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Phospho-GSK-3α/β (Tyr216/279) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Phospho-GSK-3α/β (Tyr216/279) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。