Lamin B1 Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1408
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Lamin B1
Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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70.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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1:50-1:200
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1:50-1:500
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1:50-1:200
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Lamin B1
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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4001(Human);
16906(Mouse); 116685(Rat)
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SwissProt
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P20700(Human);
P14733(Mouse); P70615(Rat)
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Synonyms
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ADLD; LMN;
LMN2; LMNB
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Category
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Apoptosis
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Background
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A unique
family of cysteine proteases has been described that differs in sequence,
structure and substrate specificity from any previously described protease
family. This family, termed Ced-3/ICE, functions as key components of the
apoptotic machinery and act to destroy specific target proteins which are
critical to cellular longevity. Nuclear lamins are critical to maintaining
the integrity of the nuclear envelope and cellular morphology as components
of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner
nuclear membrane, which is thought to provide a framework for the nuclear
envelope and may also interact with chromatin. B-type lamins undergo a series
of modifications, such as farnesylation and phosphorylation. Increased
phosphorylation of the lamins occurs before envelope disintegration and
probably plays a role in regulating lamin associations. Nuclear Lamin B is
fragmented as a consequence of apoptosis by an unidentified member of the ICE
family.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1408
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Lamin B1
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Lamin B1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。